Using The Synaptophysin Antibody Appropriately

The Synaptophysin antibody can help recognize other proteins with a molecular weight of 38kDa. It labels all normal neuroendocrine cells of the human carotid body, skin, adrenal medulla, thyroid, pituitary gland, pancreas, lung, Paneth’s cells of the gastrointestinal tract, gastrointestinal mucosa, and gastric parietal cells. It can also label neurons in the spinal cord, retina, and brain. It is designed to react with neuroendocrine neoplasms of neural and epithelial types, such as pheochromocytomas, ganglioneuromas, non-chromaffin paragangliomas, and more.

More Information

The Synaptophysin antibody has no known clone, and the immunogen is the synthetic peptide of the human protein with the same name. The isotype is the Rabbit IgG, and it has an undetermined epitope with a molecular weight of 38kDa.

Applications

The Synaptophysin antibody should be used with IHC applications. To prepare, use Formalin-fixed or paraffin-embedded tissue samples and deparaffinized slides (which can be achieved using xylene or graded alcohols). When using the concentrated version, you should dilute the antibody using a ratio of 1:300. You can also find a pre-diluted formulation that works with most protocols and methods. However, if your protocol requires a different dilution, you should use concentrated formulas only.

To retrieve the antigen, you should boil the tissue sections for ten minutes in a Citrate buffer with a pH of 6.0. Allow the antigen to cool to room temperature for 20 minutes and then incubate for ten more minutes while at room temperature.

You should wash the slides between steps using PBS/0.05 percent Tween solution. You should detect your antibody using the instruction with your detection system.

The positive control for all IHC applications includes the pancreas and pheochromocytoma. Cellular localization will always occur in the cytoplasm.

The Synaptophysin antibody can be used for researching a variety of issues. Visit Spring Bioscience today for more information.

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